Aug 01, 2001 · In eukaryotic DNA replication, replication factor-C (RFC) acts as the clamp loader, which correctly installs the sliding clamp onto DNA strands at replication forks. The eukaryotic RFC is a complex consisting of one large and four small subunits. We have determined the crystal structure of the clamp loader small subunit (RFCS) from Pyrococcus furiosus.
Learn Morenections that hold the complex together. In all clamp loaders, there is a gap between the AAA+ domains of subunits A and E (i.e. see Fig. 14.2a ). In RFC this gap is pres-ent between RFC1 and RFC5 (Fig. 14.2b and c ). In RFC, this gap is somewhat nar-rower than in the E. coli clamp loader because RFC1 has a C-terminal region that
Learn MoreJun 20, 2004 · The most recent biochemical analysis of an archaeal clamp loader showed that ATP binding by the large subunit and three of four small Communication between subunits within an archaeal clamp
Learn MoreBiochemical characterisation of the clamp/clamp loader proteins from the euryarchaeon Archaeoglobus fulgidus von Seybert, Anja in: Nucleic acids research : NAR Vol. 30, No. 20 (2013), p. 4329-4328
Learn MoreRelevant features of the bacterial clamp loader complex and of γ ATPase. (A) The sequence of events mediated by the γ clamp loader [reviewed in (53, 54)].The γ complex consists of five semicircularly arranged subunits (1 δ, 1 δ′ and 3 γ) that in the presence of ATP bind to and open the β clamp.
Learn More(C) Stylized images of the clamp loaders from bacteria (γ complex), eukaryotes (RFC), bacteriophage T4 (gp44/62), and archaea (RFC). The positions of the five subunits of each clamp loader complex are denoted by the letters A–E, with analogous subunits indicated by matching colors.
Learn MoreMay 17, 2006 · Using mutant archaeal clamp loaders deficient in either ATP binding or hydrolysis in different subunits, it was revealed that the different subunits use ATP binding and hydrolysis in distinct ways at different steps in the loading process (Seybert and Wigley, 2004). Binding of nucleotide by the large subunit and three of the four small subunits is sufficient for clamp loading.
Learn MoreApr 20, 2006 · Using mutant archaeal clamp loaders deficient in either ATP binding or hydrolysis in different subunits, it was revealed that the different subunits use ATP binding and hydrolysis in distinct ways at different steps in the loading process (Seybert and Wigley, 2004). Binding of nucleotide by the large subunit and three of the four small subunits is sufficient for clamp loading.
Learn MoreJun 20, 2004 · The eukaryotic RFC clamp loader consists of five different subunits, whereas the archaeal RFC and bacteriophage T4 loaders form similar pentamers, composed of …
Learn MoreCommunication between subunits within an archaeal clamp-loader complex Anja Seybert1, Martin R Singleton1, Nicola Cook, David R Hall and Dale B Wigley* Clare Hall Laboratories, Cancer Research UK, London ResearchInstitute, South Mimms Potters Bar, Herts, …
Learn MoreMar 16, 2006 · PubMed Abstract: We have investigated the communication between subunits in replication factor C (RFC) from Archaeoglobus fulgidus. Mutation of the proposed arginine finger in the small subunits results in a complex that can still bind ATP but has impaired clamp-loading activity, a process that normally only requires binding of nucleotide We have investigated the communication …
Learn MoreTo understand how the flat, ring-like clamps might interact with the spiral interaction surface of the clamp loader complex, we have performed molecular dynamics simulations of sliding clamps (proliferating cell nuclear antigen from the budding yeast, humans, and an archaeal species) in which we have removed one of the three subunits so as to
Learn MoreUsing mutant archaeal clamp loaders deficient in either A TP binding or hydroly sis in different subunits, it was revea led that the different subunits use A TP binding and
Learn MoreJul 27, 2018 · Seybert A, Singleton MR, Cook N, Hall DR, Wigley DB (2006) Communication between subunits within an archaeal clamp-loader complex. EMBO J 25:2209–2218. CAS Article PubMed PubMed Central Google Scholar 19.
Learn MoreE. coli clamp loader is sho wn at the right of Fig. 14.2a, to illustrate the three domains structure of clamp loader subunits (Jeruzalmi et al. 2001a ) . ention, By v conclamp loaders are viewed from the ÒsideÓ with the C-terminal domain at the top, and the N-terminal AAA+ domains at …
Learn MoreThe residues on the loops that extend from the β clamp and bind DNA result in a pronounced tilt of the clamp on DNA, and this tilt may help disconnect the clamp from some of the subunits of the clamp loader (the first step in Fig. 14.5a). One may presume that the last connection between the clamp loader and clamp to be disrupted is the tight
Learn MoreHingorani,M.M. and Coman,M.M. (2002) On the specificity of interaction between the Saccharomyces cerevisiae clamp loader replication factor C and primed DNA templates during DNA replication. J Biol Chem 277, 47213-47224. 3.
Learn MoreDec 26, 2020 · Structural analysis of a eukaryotic sliding DNA clamp-clamp loader complex. Nature429:724 Communication between subunits within an archaeal clamp-loader complex. EMBO J.25 Distinct roles for ATP binding and hydrolysis at individual subunits of an archaeal clamp loader. EMBO J.23:1360-1371. PubMed. Google Scholar. 106. Shechter, D., C. Y
Learn MoreMar 11, 2004 · Results and discussion. The aim of this study was to analyse nucleotide binding to the A. fulgidus clamp loader and the functional role of bound nucleotide. Sequence alignments show that both the large and small afRFC subunits belong to the AAA+ family (Neuwald et al, 1999) and have a number of conserved sequence motifs.Notably, two motifs critical for nucleotide hydrolysis and binding, …
Learn MoreOct 12, 2008 · Seybert, A. & Wigley, D.B. Distinct roles for ATP binding and hydrolysis at individual subunits of an archaeal clamp loader. EMBO J. 23, 1360–1371 (2004). CAS Article Google Scholar
Learn More